Application
Immunofluorescence staining of CD71 in human HeLa cell line using anti-CD71 (MEM-75; green;). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).Application
Surface staining of NALM-6 human peripheral blood pre-B cell leukemia cell line with anti-CD71 (MEM-75): Biotin ; detection by Streptavidin-PE . Total viable cells were used for analysis.| Product Name | CD71 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [MEM-75] to CD71Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. |
| Immunogen | Pre-B cell line NALM-6. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-75 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIP |
| FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | TFR TFR1 7037 190010 Transferrin receptor TFRC CD71 P02786 TRFR |
|---|---|
| Target | CD71 |
| Tissue Specificity | Human CD71 (transferrin receptor). This antibody does not block the binding of transferrin to the receptor. |
Application
Immunofluorescence staining of CD71 in human HeLa cell line using anti-CD71 (MEM-75; green;). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Application
Surface staining of NALM-6 human peripheral blood pre-B cell leukemia cell line with anti-CD71 (MEM-75): Biotin ; detection by Streptavidin-PE . Total viable cells were used for analysis.| Application Notes | FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
