Application
Formalin fixed paraffin embedded human tonsil stained with CD74, B cell antibody (cat. # ).| Product Name | CD74 antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Human CD74This antibody reacts with a 35 kD antigen present on the nuclear membrane protein related to the invariant chain of the HLA-Dr antigen. This antibody reacts with the germinal center and mantle zone B lymphocytes, interdigitating histiocytes in T cell zones and mononuclear cells and Reed-Sternberg cells in Hodgkin's disease. This antibody may be useful in the identification of germinal center derived B cell lymphomas and leukemias. |
| Immunogen | SU-DHL-4 lymphoma cells |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | LN2 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-FIHC-P |
| Recommended Starting Dilutions: For IHC-P: Use at a dilution of 1:25-1:50. Suggested antigen retrieval with 10 mM citrate buffer, pH 6.0 prior to immunostaining. For ICC/IF: Use at a assay dependent dilution. For FACS: Use at a assay dependent dilution. Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | protein41 Ia-GAMMA IaGAMMA 972 142790 P04233 DHLAG HLADG protein 41 CD74 II Ia GAMMA |
|---|---|
| Target | CD74 |
| Cellular Localization | Cell membrane |
| Light Chain Type | Kappa |
Application
Formalin fixed paraffin embedded human tonsil stained with CD74, B cell antibody (cat. # ).| Positive Control | Tonsil |
|---|---|
| Application Notes | Recommended Starting Dilutions: For IHC-P: Use at a dilution of 1:25-1:50. Suggested antigen retrieval with 10 mM citrate buffer, pH 6.0 prior to immunostaining. For ICC/IF: Use at a assay dependent dilution. For FACS: Use at a assay dependent dilution. Optimal dilutions should be determined experimentally by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | The antibody contains less than 0.1% sodium azide. |
