Application
Formalin fixed paraffin embedded human tonsil stained with CD8 antibody (cat. # ).| Product Name | CD8 antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [144B] to CD8 T cell (ready-to-use) |
| Immunogen | Synthetic peptide comprising of 13 C terminal amino acids ofthe cytoplasmic domain of CD8 molecule. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 144B |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| For IHC-P: Pretitered and quality controlled to work on formalin-fixed paraffin-embedded. No further titration is required. For IHC-Fr: Pretitered and quality controlled to work on acetone fixed frozen tissue sections. No further titration is required. Incubation period of 30-60 minutes at room temperature or overnight at 4oC is recommended. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Target | CD8 |
|---|---|
| Tissue Specificity | Reacts specifically with a 32 kD protein similar to CD8 molecule. This antibody stains cytotoxic/suppressor T cells and shows a staining patterns similar to clustered CD8 monoclonal antibodies. |
| Cellular Localization | Cell membrane |
| Light Chain Type | kappa |
Application
Formalin fixed paraffin embedded human tonsil stained with CD8 antibody (cat. # ).| Positive Control | Tonsil |
|---|---|
| Application Notes | For IHC-P: Pretitered and quality controlled to work on formalin-fixed paraffin-embedded. No further titration is required. For IHC-Fr: Pretitered and quality controlled to work on acetone fixed frozen tissue sections. No further titration is required. Incubation period of 30-60 minutes at room temperature or overnight at 4oC is recommended. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | PBS, pH7.2 containing 0.05% sodium azide |
