| Product Name | CD8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [B334 (H030-1.2)] to CD8 T-cellMonoclonal Mouse Anti-Human T-cell, CD8 |
| Immunogen | E-rosette forming cells purified from human PBL |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | B334 (H030-1.2) |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-F |
| Immunofluorescence 5-20 ug/mL. Flow cytometry 1-5 ug/10^6 cells. Immunohistochemistry 5-20 ug/mL on frozen sections using avidin-biotin system. The optimal dilution factors should be determined by the individual laboratory. This antibody was not quality controlled in flow cytometry. This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 186910 P10966 MGC119115 926 LYT3 p32 Ly3 CD8 CD8B 925 MAL Leu2 186730 P01732 CD8B1 CD8A |
|---|---|
| Target | CD8 |
| Tissue Specificity | This antibody reacts with a 32 kD molecule present on human suppressor/cytotoxic T-cells. These antibodies also react with sinus-lining cells in the human spleen. |
| Positive Control | Tonsil and Lymph Node |
|---|---|
| Application Notes | Immunofluorescence 5-20 ug/mL. Flow cytometry 1-5 ug/10^6 cells. Immunohistochemistry 5-20 ug/mL on frozen sections using avidin-biotin system. The optimal dilution factors should be determined by the individual laboratory. This antibody was not quality controlled in flow cytometry. This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.2% BSA and 0.09% sodium azide |
