Application
Surface staining of human peripheral blood lymphocytes using anti-human CD8: PE .| Product Name | CD8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM-31] to CD8The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain isoforms. Two alternative transcripts encoding distinct isoforms, one membrane associated and one secreted, have been identified. |
| Immunogen | Tissue / cell preparation (Human). (Crude thymus membrane fraction). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | MEM-31 |
| Host Species | Mouse |
| Tested Applications | FACSIP |
| FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. Has not been shown to work in IHC-F or WB. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 186910 P10966 MGC119115 926 LYT3 p32 Ly3 CD8 CD8B 925 MAL Leu2 186730 P01732 CD8B1 CD8A |
|---|---|
| Target | CD8 |
| Tissue Specificity | CD8 antigen (human). |
| Cellular Localization | Cell Membrane |
Application
Surface staining of human peripheral blood lymphocytes using anti-human CD8: PE .| Application Notes | FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. Has not been shown to work in IHC-F or WB. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
