Application
IHC-Fr ImageFrozen section of human tonsil stained with Monoclonal Anti-CD8, clone no. UCHT-4 using the APAAP method.| Product Name | CD8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [UCHT-4] to CD8The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain isoforms. Two alternative transcripts encoding distinct isoforms, one membrane associated and one secreted, have been identified. |
| Immunogen | Human thymocytes followed by peripheral blood T cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | UCHT-4 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-F |
| For FACS: Use at a concentration of 5 μl using 1x10^6 cells. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein A |
| Gene Synonyms | 186910 P10966 MGC119115 926 LYT3 p32 Ly3 CD8 CD8B 925 MAL Leu2 186730 P01732 CD8B1 CD8A |
|---|---|
| Target | CD8 |
| Cellular Localization | Cell Membrane |
Application
IHC-Fr ImageFrozen section of human tonsil stained with Monoclonal Anti-CD8, clone no. UCHT-4 using the APAAP method.| Application Notes | For FACS: Use at a concentration of 5 μl using 1x10^6 cells. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide |
