| Product Name | CD81 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [B399 1.3.3.22] to CD81TAPA1, or CD81, is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell surface proteins that are characterised by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TAPA1 is a cell surface glycoprotein that is known to complex with integrins. It appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. TAPA1 gene is localized in the tumor suppressor gene region and thus it is a candidate gene for malignancies. |
| Immunogen | Burkitt’s lymphoma cell line |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | B399 1.3.3.22 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-FWB |
| For IF: Use at 10-20 ug/ml. For FACS: Use 1ug for 10^6 cells. For WB: Use at 1-5ug/ml. This antibody can be used on frozen tissue sections only. On formalin-fixed, paraffin embedded tissues, some weak positive staining is observed when treated with Auto/Zyme. Used for identification of eosinophils from a granulocyte subpopulation. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | S5.7 186845 TAPA1 Q5U0J6 TSPAN28 975 CD81 CVID6 |
|---|---|
| Target | CD81 |
| Tissue Specificity | This antibody reacts with a 24 kD protein, a member of the tetraspan family, with both termini in the cytoplasm and two loops of extracellular protein. This protein lacks external N-glycosylation sites. |
| Positive Control | For IHC:Tonsil sections & WB:Raji, Jurkat |
|---|---|
| Application Notes | For IF: Use at 10-20 ug/ml. For FACS: Use 1ug for 10^6 cells. For WB: Use at 1-5ug/ml. This antibody can be used on frozen tissue sections only. On formalin-fixed, paraffin embedded tissues, some weak positive staining is observed when treated with Auto/Zyme. Used for identification of eosinophils from a granulocyte subpopulation. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.2% BSA and 0.09% sodium azide |
