Application
Formalin-fixed and paraffin-embedded human brain tissue with CD81 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of CD81 Antibody (C-term) in mouse kidney(lane 1) and cerebellum(lane 2) tissue lysates (35ug/lane). CD81 (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of 293 cells using CD81 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CD81 antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CD81 (C-term)The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 182~212 amino acids from the C-terminal region of human CD81. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Target of the antiproliferative antibody1 Tetraspanin28 26 kDa cell surface protein TAPA-1 Target of the antiproliferative antibody 1 CD81 CD81 antigen Tspan-28 TAPA1 26 kDa cell surface protein TAPA1 Tetraspanin-28 TSPAN28 Tspan 28 Tetraspanin |
|---|---|
| Target | CD81 |
| Molecular Weight(MW) | 25.809 kDa (note) |
Application
Formalin-fixed and paraffin-embedded human brain tissue with CD81 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of CD81 Antibody (C-term) in mouse kidney(lane 1) and cerebellum(lane 2) tissue lysates (35ug/lane). CD81 (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of 293 cells using CD81 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
