Application
ApplicationApplication
Formalin-fixed, paraffin-embedded human tonsil stained with using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of lymphocytes in the germinal center.| Product Name | CDC2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal [A17.1.1] to CDC2p34cdc2(a catalytic subunit of Maturation Promoting Factor) plays a crucial role during cell division and is most active during mitosis. It is a serine/threonine kinase, which is activated by cyclin, by dephosphorylation of tyrosine residues. |
| Immunogen | C-Terminal 2/3rds of Xenopus cdc2 expressed in E.coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | A17.1.1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PInhibitionIPWB |
| For IF: Use at an assay dependent dilution. For IHC-P: Use at a concentration of 2-4μg/ml for 30min at RT. For Inhib (Inhibits activation of p34cdc2 kinase by cyclins): Use at an assay dependent dilution. For IP (Native and denatured): Use at a concentartion of 2μg/mg lysate. For WB: Use at a concentration of 2-4μg/ml for 30 min at RT. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMousePigRatGuinea PigWoodchuckXenopus |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | CDK1 |
|---|---|
| Cellular Localization | Cytoplasmic |
Application
Application
Application
Formalin-fixed, paraffin-embedded human tonsil stained with using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of lymphocytes in the germinal center.| Positive Control | HeLa, MAD109, PC12 or NIH-3T3 Cells, Tonsil, or Lymph Node |
|---|---|
| Application Notes | For IF: Use at an assay dependent dilution. For IHC-P: Use at a concentration of 2-4μg/ml for 30min at RT. For Inhib (Inhibits activation of p34cdc2 kinase by cyclins): Use at an assay dependent dilution. For IP (Native and denatured): Use at a concentartion of 2μg/mg lysate. For WB: Use at a concentration of 2-4μg/ml for 30 min at RT. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, no preservative added. |
