Application
Western blot analysis using CDC27 mouse mAb against CDC27 (AA: 724-830)-hIgGFc transfected HEK293 cell.Application
Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and colon cancer tissues (right) using CDC27 mouse mAb with DAB staining.| Product Name | CDC27 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5C12] to CDC27The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human CDC27 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5C12 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NUC2 D0S1430E CDC27HS HNUC D17S978E ANAPC3 APC3 |
|---|---|
| Target | CDC27 |
| Molecular Weight(MW) | 91 kDa (note) |
Application
Western blot analysis using CDC27 mouse mAb against CDC27 (AA: 724-830)-hIgGFc transfected HEK293 cell.
Application
Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and colon cancer tissues (right) using CDC27 mouse mAb with DAB staining.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
