Application
Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-Cdc27 Clone: AF3.1 . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. MDBK 3. COS7 4. NIH-3T3 5. P19| Product Name | CDC27 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [AF3.1] to Cdc27Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to amino acids 814-823 of Human Cdc27. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | AF3.1 |
| Host Species | Mouse |
| Tested Applications | PurifyELISAIHC-FIHC-PWB |
| AP: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 97 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDogRatXenopusBovine |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | 116946 CDC27 P30260 D17S978E 996 HNUC APC3 D0S1430E |
|---|---|
| Target | CDC27 |
Application
Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-Cdc27 Clone: AF3.1 . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. MDBK 3. COS7 4. NIH-3T3 5. P19| Positive Control | HeLa cell nuclear extract. |
|---|---|
| Application Notes | AP: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 97 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
