Application
Sample (30 µg of whole cell lysate) A: JurKat 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Cdc6 antibody detects CDC6 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 7.5% SDS-PAGE Cdc6 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Cdc6 antibody detects CDC6 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg BCL-1 whole cell lysate/extract E. 30 µg Raw264.7 whole cell lysate/extract F. 30 µg C2C12 whole cell lysate/extract 7.5% SDS-PAGE Cdc6 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cdc6 antibody at 1:200 dilution.| Product Name | Cdc6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cdc6 (cell division cycle 6 homolog (S. cerevisiae))The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cdc6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (89%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDC18L antibody HsCDC18 antibody HsCDC6 antibody CDC6 antibody p62(cdc6) antibody cdc18-related protein antibody CDC6-related protein antibody CDC6 cell division cycle 6 homolog antibody cell division control protein 6 homolog antibody cell divisi |
|---|---|
| Molecular Weight(MW) | 63 kDa (note) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Sample (30 µg of whole cell lysate) A: JurKat 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Cdc6 antibody detects CDC6 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 7.5% SDS-PAGE Cdc6 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Cdc6 antibody detects CDC6 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg BCL-1 whole cell lysate/extract E. 30 µg Raw264.7 whole cell lysate/extract F. 30 µg C2C12 whole cell lysate/extract 7.5% SDS-PAGE Cdc6 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cdc6 antibody at 1:200 dilution.| Positive Control | Jurkat , Neuro2A , GL261 , C8D30 , BCL-1 , Raw264.7 , C2C12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
