Application
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE diluted at 1:1000Application
CDC6 antibody detects CDC6 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDC6 protein stained by CDC6 antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using CDC6 antibody at 1:250 dilution.| Product Name | CDC6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDC6 (cell division cycle 6 homolog (S. cerevisiae))The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CDC6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDC18L antibody HsCDC18 antibody HsCDC6 antibody CDC6 antibody p62(cdc6) antibody cdc18-related protein antibody CDC6-related protein antibody CDC6 cell division cycle 6 homolog antibody cell division control protein 6 homolog antibody cell divisi |
|---|---|
| Molecular Weight(MW) | 63 kDa (note) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE diluted at 1:1000
Application
CDC6 antibody detects CDC6 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDC6 protein stained by CDC6 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using CDC6 antibody at 1:250 dilution.| Positive Control | NT2D1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
