Application
HeLa cell (human) extract and Human uterine mesodermal tumor cell-SKUT extract were separated on SDS-PAGE and probed with Monoclonal Anti-CDK 4, clone DCS-31 (Cat. No. ). The antibody was developed with Anti-Mouse IgG (Fab), alkaline phosphatase and a NBT/BCIP substrate. Antibody dilution 1:2,000.| Product Name | CDK4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [DCS - 31] to Cdk4Regulation of cell cycle progression in eukaryotic cells depends on the expression of cyclins. These proteins form complexes with several different cyclin dependant kinases (CDKs). Within complexes, the cyclin subunit serves a regulatory role, whereas the CDKs have a catalytic protein kinase activity. Complexes of cyclins and CDKs play a key role in cell cycle control. The eukaryotic cell cycle is regulated by the sequential activation of CDKs. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | DCS-31 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| ICC: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. (recognises a 33kD band, and it may pick up additional weaker bands, using a cultured human tumor cell line extract) Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P11802 1019 CDK4 123829 PSKJ3 PSK-J3 CMM3 MGC14458 PSK J3 |
|---|---|
| Target | CDK4 |
| Tissue Specificity | Reacts specifically with Cdk4. Does not recognise other Cdk types. |
| Cellular Localization | Cytoplasmic (likely) |
Application
HeLa cell (human) extract and Human uterine mesodermal tumor cell-SKUT extract were separated on SDS-PAGE and probed with Monoclonal Anti-CDK 4, clone DCS-31 (Cat. No. ). The antibody was developed with Anti-Mouse IgG (Fab), alkaline phosphatase and a NBT/BCIP substrate. Antibody dilution 1:2,000.| Application Notes | ICC: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. (recognises a 33kD band, and it may pick up additional weaker bands, using a cultured human tumor cell line extract) Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Ascitic fluid with 15mM sodium azide |
