| Product Name | CDK5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [DC17] to p31 Cdk5Cdk 5 is expressed in both cycling and non cycling cells and is present in numerous mammalian tissues. Neuronal and muscle cells contain the highest amount of this protein. The p35 protein, which is expressed solely in the brain, activates Cdk 5. Cdk 5 activity is involved in terminal differentiation of neurons and muscle cells. |
| Immunogen | Recombinant full length protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | DC17 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PIPWB |
| For IF: Use at an assay dependent dilution. For IHC-P: Use at a dilution of 1:200 for 30 min at RT. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 min. For IP (native and denatured): Use at a dilution of 1:200 of protein lysate (Use protein G). For WB: Use at a dilution of 1:100. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Cell Division Kinase 5 Cyclin Dependent Kinase 5 PSSALRE |
|---|---|
| Target | CDK5 |
| Cellular Localization | Nuclear and cytoplasmic. |
| Positive Control | HT29 or MAD109 cells. Brain |
|---|---|
| Application Notes | For IF: Use at an assay dependent dilution. For IHC-P: Use at a dilution of 1:200 for 30 min at RT. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 min. For IP (native and denatured): Use at a dilution of 1:200 of protein lysate (Use protein G). For WB: Use at a dilution of 1:100. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 10mM Phosphate-buffered saline, pH7.4 containing 0.2%BSA and 0.09% sodium azide |
