Mouse monoclonal [B360 (LN-1)] to Cdw75Human CDw75 cell surface antigen, an alpha 2.6 - sialylated carbohydrate epitope, is expressed by mature B cells (especially germinal centre B cells), red blood cells and some epithelial cells.
Immunogen
Human lymphocytes and SU-DHL4 cell line
Key Feature
Clonality
Monoclonal
Isotype
IgM
Clone Number
B360 (LN-1)
Host Species
Mouse
Tested Applications
FACSICC/IFIHC-FIHC-PWB
For IF: Use at a concentration of 5-20 ug/mL. For FACS: Use at a concentration of 1-5 ug/10^6 cells. For WB: Use at a concentration of 1-5 ug/mL. For IHC: Use at aconcentration of 5-10mg/ml. Prolonged fixation is buffered formalin can destroy the epitope. Optimal dilutions/concentrations should be determined by the end user.:
Species Reactivity
Human
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
Cdw75 antibody CD75 antibody
Target
Cdw75
Tissue Specificity
This antibody reacts with Cdw75, a cell surface sialoglycan that is neuraminidase sensitive. Cdw75 has a function in all adhesion and is the ligand for CD22.
Application Notes
For IF: Use at a concentration of 5-20 ug/mL. For FACS: Use at a concentration of 1-5 ug/10^6 cells. For WB: Use at a concentration of 1-5 ug/mL. For IHC: Use at aconcentration of 5-10mg/ml. Prolonged fixation is buffered formalin can destroy the epitope. Optimal dilutions/concentrations should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.