Application
Immunohistochemical analysis of paraffin-embedded Human colon, using CEA antibody antibody at 1:100 dilution.| Product Name | CEA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [Col-1] to Carcino Embryonic Antigen CEACarcino Embryonic Antigen (CEA) is synthesised during development in the fetal gut, and is re expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). |
| Immunogen | Colon carcinoma cells extract |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | Col-1 |
| Host Species | Mouse |
| Tested Applications | IHC-PIPWB |
| IHC-P: Use at a dilution of 1:25-1:80. An incubation period of 30 minutes at room temperature is recommended. Enzymatic predigestion of paraffin embedded tissues may be required before immunostaining. For IP: Use at a dilution of 1:50. For WB: Use at a dilution of 1:50-1:100. Predicted molecular weight: 77 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | P06731 CEACAM5 Cea 114890 111518 CD66e 1048 DKFZp781M2392 |
|---|---|
| Target | CEA |
| Tissue Specificity | This antibody stains specific regions of CEA. It reacts with colorectal adenocarcinomas but does not show any staining with polymorphonuclear neutrophils and erythrocytes. |
| Cellular Localization | Attached to the membrane by a GPI-anchor. |
| Light Chain Type | kappa |
Application
Immunohistochemical analysis of paraffin-embedded Human colon, using CEA antibody antibody at 1:100 dilution.| Positive Control | Colon Carcinoma |
|---|---|
| Application Notes | IHC-P: Use at a dilution of 1:25-1:80. An incubation period of 30 minutes at room temperature is recommended. Enzymatic predigestion of paraffin embedded tissues may be required before immunostaining. For IP: Use at a dilution of 1:50. For WB: Use at a dilution of 1:50-1:100. Predicted molecular weight: 77 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS with 1% BSA and 0.05% sodium azide as preservative |
