Application
Formalin fixed paraffin embedded human colon carcinoma stained with CEA antibody .| Product Name | CEA antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [Col-1] to Carcino Embryonic Antigen CEA, predilutedCarcino Embryonic Antigen (CEA) is synthesised during development in the fetal gut, and is re expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). |
| Immunogen | BALB/C mice were injected with extract of colon carcinoma cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | Col-1 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| IHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution (acetone fixed sections). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | P06731 CEACAM5 Cea 114890 111518 CD66e 1048 DKFZp781M2392 |
|---|---|
| Target | CEA |
| Tissue Specificity | This antibody reacts with a highly glycosylated protein of MW 180 kD, synthesized during development in the fetal gut and several tumors. It does not react with normal colon antigen (NCA). It stains the cell surface and cytoplasm of malignant glands in colorectal adenocarcinomas. |
| Cellular Localization | Attached to the membrane by a GPI-anchor. |
| Light Chain Type | kappa |
Application
Formalin fixed paraffin embedded human colon carcinoma stained with CEA antibody .| Positive Control | Colon carcinoma |
|---|---|
| Application Notes | IHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution (acetone fixed sections). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
