| Product Name | CENPF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CENP - FThe kinetophore is a macromolecular structure that is associated with the centromeres of chromosomes and is responsible for establishing and maintaining the connections with the microtubules of the mitotic spindle. The proteins that reside within the chromosomal domain fall into two broad classes: those that associate with the centromere throughout the cell cycle and those that are found at the centromere in a cell cycle specific manner. Members of the first class include CENP-A, CENP-B, and CENP-C. The members of the second class include several ubiquitous cellular proteins such as calmodulin, tubulin, dynein and p34 cdc2 and also CENP-E and CENP-F. CENP-F appears to be the earliest member to interact with the centromere-kinetochore complex. CENP-F also shows a cell-cycle distribution that is both temporally regulated and diverse in terms of the structural components it localizes to and therefore may play a role in several mitotic events. (1-3) |
| Immunogen | A bacterial fusion protein from the C-terminus of CENP-F. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| For WB: Use at a dilution of 1:500. By western blot a band of ~ 330 kDa is seen. For IF: Use at a dilution of 1:750. For IP: Use at a dilution of 1:50. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 600236 1063 CENPF PRO1779 CENF P49454 hcp-1 hcp 1 hcp1 |
|---|---|
| Target | CENPF |
| Tissue Specificity | This antibody is specific for CENP-F. |
| Cellular Localization | As cells progress through S phase, weak nuclear staining is observed. In G2 phase, very bright CENP-F staining is detected uniformly throughout the nucleus but excluded from the nucleoli. |
| Application Notes | For WB: Use at a dilution of 1:500. By western blot a band of ~ 330 kDa is seen. For IF: Use at a dilution of 1:750. For IP: Use at a dilution of 1:50. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline |
