Application
Confocal immunofluorescence staining (Olympus FV10i) of Mitosin. U2OS cells were fixed by 4% PFA and costained with Mitosin 14C10 1D8 AB (Red; cat# 1:500 Ab dilution) and alpha-tubulin rabbit polyclonal AB (Green; cat# 1:500 Ab dilution ), a spindle marker. DAPI (blue), chromosomes. Scale bar, 5 umApplication
CENPF antibody [14C10 1D8] detects CENPF protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. CENPF antibody [14C10 1D8] dilution: 1:200.Application
CENPF antibody [14C10 1D8] detects CENPF protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract (untreated) B. 30 µg HeLa whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr) 5% SDS-PAGE CENPF antibody [14C10 1D8] dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | CENPF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [14C10 1D8] to CENPFMitosin-14C10 recognizes mitosin, a 350kDa phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase, and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation. |
| Immunogen | Protein fragment expressed in E. coli corresponding to amino acids 1759-2093. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 14C10 1D8 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-FIHC-PIPWB |
| For IHC-Fr: Use at an assay dependent dilution. For IHC-P: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:500. Predicted molecular weight: 350 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CENF PRO1779 hcp-1 1063 CENPF CENP F AH Antigen 1063 CENPF CENPF AH Antigen |
|---|---|
| Target | Mitosin |
| Molecular Weight(MW) | 350 kDa (note) |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
Application
Confocal immunofluorescence staining (Olympus FV10i) of Mitosin. U2OS cells were fixed by 4% PFA and costained with Mitosin 14C10 1D8 AB (Red; cat# 1:500 Ab dilution) and alpha-tubulin rabbit polyclonal AB (Green; cat# 1:500 Ab dilution ), a spindle marker. DAPI (blue), chromosomes. Scale bar, 5 um
Application
CENPF antibody [14C10 1D8] detects CENPF protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. CENPF antibody [14C10 1D8] dilution: 1:200.
Application
CENPF antibody [14C10 1D8] detects CENPF protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract (untreated) B. 30 µg HeLa whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr) 5% SDS-PAGE CENPF antibody [14C10 1D8] dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | HeLa (G2 phase) |
|---|---|
| Application Notes | For IHC-Fr: Use at an assay dependent dilution. For IHC-P: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:500. Predicted molecular weight: 350 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing no preservatives |
