Application
Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE diluted at 1:1000Application
Cep 164 antibody detects Cep 164 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Cep 164 antibody diluted by 1:10000.| Product Name | CEP164 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [17] to Cep 164This monoclonal antibody binds to Cep164 or centrosonal protein 164kDa. Cep164 plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Plays a critical role in G2/M checkpoint and nuclear divisions. A key player in the DNA damage-activated ATR/ATM signaling cascade since it is required for the proper phosphorylation of H2AX, RPA,CHK2 and CHK1. Plays a critical role in chromosome segregation, acting as a mediator required for the maintenance of genomic stability through modulation of MDC1, RPA and CHK1. [from UniProt] |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 17 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| Suggested starting dilutions are as follows: WB: 1:500-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | KIAA1052 CEP164 Cep 164 centrosomal protein164kDa centrosomal protein 164kDa |
|---|---|
| Target | Cep 164 |
| Cellular Localization | Localizes specifically to very distally located appendage structures on the mature centriole from which initiate PC formation. Persisted at centrioles throughout mitosis. Expressed in chromatin-enriched nuclear fraction of HeLa cells. |
Application
Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE diluted at 1:1000
Application
Cep 164 antibody detects Cep 164 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Cep 164 antibody diluted by 1:10000.| Positive Control | HeLa , A549 |
|---|---|
| Application Notes | Suggested starting dilutions are as follows: WB: 1:500-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short term use, store at 4°C. For extended use, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS |
