Application
Western blot analysis of extracts from K562 cells, treated with etoposide 25uM 1h, using CFAB Bb (Cleaved-Lys260) Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | CFAB Bb (cleaved Lys260) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CFAB Bb (cleaved Lys260)Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CFAB Bb. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:20000Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CFAB Bb FB GBG AHUS4 FBI12 BFD CFAB BF H2Bf PBF2 CFAB Bb (cleaved K260) CFAB Bb (cleaved Lys260) CFAB Bb cleaved K260 CFAB Bb cleaved Lys260 Cleaved CFAB Bb (K260) Cleaved CFAB Bb (Lys260) |
|---|---|
| Target | CFAB Bb |
| Molecular Weight(MW) | 57 and 85 kDa (note) |
| Tissue Specificity | CFAB Bb (Cleaved-Lys260) Antibody detects endogenous levels of fragment of activated CFAB Bb resulting from cleavage adjacent to Lys260. |
Application
Western blot analysis of extracts from K562 cells, treated with etoposide 25uM 1h, using CFAB Bb (Cleaved-Lys260) Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:20000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
