Application
c-Fos antibody detects c-Fos protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: c-Fos protein stained by c-Fos antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.Application
c-Fos antibody detects FOS protein by Western blot analysis. A. 30 µg MCF-7 whole cell lysate/extract (serum starvation) B. 30 µg MCF-7 whole cell lysate/extract (500nM PMA treatment for 0.5hr) C. 30 µg MCF-7 whole cell lysate/extract (500nM PMA treatment for 1hr) D. 30 µg MCF-7 whole cell lysate/extract (500nM PMA treatment for 2hr) 10 % SDS-PAGE c-Fos antibody dilution: 1:5000| Product Name | c-Fos antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to c-Fos (FBJ murine osteosarcoma viral oncogene homolog)The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human c-Fos. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cat, Pig, Sheep, Rhesus Monkey, Chimpanzee, BovineMouse (94%), Rat (94%), Cat (97%), Pig (96%), Sheep (95%), Rhesus Monkey (99%), Chimpanzee (100%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AP-1 antibody C-FOS antibody FOS antibody proto-oncogene c-Fos antibody FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS) antibody cellular oncogene c-fos antibody G0/G1 switch regulatory protein 7 antibody activator protein 1 an |
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| Molecular Weight(MW) | 41 kDa (note) |
| Cellular Localization | Nucleus |
Application
c-Fos antibody detects c-Fos protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: c-Fos protein stained by c-Fos antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.
Application
c-Fos antibody detects FOS protein by Western blot analysis. A. 30 µg MCF-7 whole cell lysate/extract (serum starvation) B. 30 µg MCF-7 whole cell lysate/extract (500nM PMA treatment for 0.5hr) C. 30 µg MCF-7 whole cell lysate/extract (500nM PMA treatment for 1hr) D. 30 µg MCF-7 whole cell lysate/extract (500nM PMA treatment for 2hr) 10 % SDS-PAGE c-Fos antibody dilution: 1:5000| Positive Control | MCF-7 treat 500nM PMA 0.5hr , MCF-7 treat 500nM PMA 1hr , MCF-7 treat 500nM PMA 2hr |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
