| Product Name | c-Fos antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2G9C3] to c-FosThe Fos gene family consists of 4 members: Fos, FosB, FosL1, and FosL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP1. As such, the Fos proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the Fos gene has also been associated with apoptotic cell death. The human oncogene c-Fos is homologous to the Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus oncogene. Fos was the first transcription factor identified that has a critical function in regulating the development of cells destined to form and maintain the skeleton. |
| Immunogen | Synthetic peptide: KVEQLSPEEEEKRRIRRERNKMAAA, corresponding to Internal sequence amino acids 128 - 152 of Human c-Fos. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 2G9C3 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| Not suitable for IHC frozen or parraffin-embedded sections. For ICC: Use at a concentration of 2.5 - 5.0 µg/ml. For WB: Use at a concentration of 2.5 - 5.0 µg/ml. Detects bands of approximately 55 and 62 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Gene Synonyms | 2353 FOS 164810 c-fos cFos P01100 c Fos |
|---|---|
| Target | c-Fos |
| Molecular Weight(MW) | 55 - 62 kD (note) |
| Tissue Specificity | Reacts with both cellular and viral forms, will detect both the 55 kDa and 62 kDa c-fos and v-fos proteins, but does not react with the 39 kDa jun protein. |
| Cellular Localization | Nuclear |
| Positive Control | HeLa cells |
|---|---|
| Application Notes | Not suitable for IHC frozen or parraffin-embedded sections. For ICC: Use at a concentration of 2.5 - 5.0 µg/ml. For WB: Use at a concentration of 2.5 - 5.0 µg/ml. Detects bands of approximately 55 and 62 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5 with ²0.1% sodium azide |
