Application
Flow cytometric analysis of K562 cells using CHD3 mouse mAb (green) and negative control (purple).Application
Western blot analysis using CHD3 mouse mAb against Hela (1), K562 (2), Jurkat (3), NTERA-2 (4), HEK293 (5), Raji (6) cell lysate and mouse brain (7) tissue lysate.Application
Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CHD3 mouse mAb with DAB staining.Application
Immunofluorescence analysis of Hela cells using CHD3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.| Product Name | CHD3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2G4] to CHD3This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human CHD3 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2G4 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Synonyms | ZFH MI2-ALPHA MI-2A |
|---|---|
| Target | CHD3 |
| Molecular Weight(MW) | 226 kDa (note) |
Application
Flow cytometric analysis of K562 cells using CHD3 mouse mAb (green) and negative control (purple).
Application
Western blot analysis using CHD3 mouse mAb against Hela (1), K562 (2), Jurkat (3), NTERA-2 (4), HEK293 (5), Raji (6) cell lysate and mouse brain (7) tissue lysate.
Application
Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CHD3 mouse mAb with DAB staining.
Application
Immunofluorescence analysis of Hela cells using CHD3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Purified antibody in PBS containing 0.03% sodium azide. |
