Application
FACS analysis of K562 cells using CHD3 antibody at a dilution of 1:100 (red) or an isotype control (black).Application
WB analysis of Y79 and Raji lysates using CHD3 antibody at a dilution of 1:1,000.Application
IHC-P analysis of human colon tissue using CHD3 antibody.| Product Name | CHD3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [4B1-F1-G4] to CHD3This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. |
| Immunogen | Purified recombinant human CHD3 protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4B1-F1-G4 |
| Host Species | Mouse |
| Tested Applications | FACSIHCIHC-PWB |
| FACS:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Mi2-ALPHA Mi-2a chromodomain helicase DNA binding protein 3 ZFH |
|---|---|
| Target | CHD3 |
| Molecular Weight(MW) | 260kDa (note) |
| Cellular Localization | Nucleus. Cytoplasm |
Application
FACS analysis of K562 cells using CHD3 antibody at a dilution of 1:100 (red) or an isotype control (black).
Application
WB analysis of Y79 and Raji lysates using CHD3 antibody at a dilution of 1:1,000.
Application
IHC-P analysis of human colon tissue using CHD3 antibody.| Application Notes | FACS:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol |
