Application
WB analysis of various cell lysates using CHD3 antibody [7F6-G2-E5] at a dilution of 1:1000.Application
IP analysis with HeLa lysates and either control IgG or CHD3 antibody [7F6-G2-E5]. The precipitates were detected by the same antibody.Application
ICC/IF analysis of HeLa cells using CHD3 antibody [7F6-G2-E5] at a dilution of 1:400.| Product Name | CHD3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [7F6-G2-E5] to CHD3This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| Immunogen | Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7F6-G2-E5 |
| Host Species | Mouse |
| Tested Applications | IDICC/IFIPWB |
| ICC/IF:1:200~1:500 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | Mi2-ALPHA chromodomain helicase DNA binding protein 3 ZFH Mi-2a |
|---|---|
| Target | CHD3 |
Application
WB analysis of various cell lysates using CHD3 antibody [7F6-G2-E5] at a dilution of 1:1000.
Application
IP analysis with HeLa lysates and either control IgG or CHD3 antibody [7F6-G2-E5]. The precipitates were detected by the same antibody.
Application
ICC/IF analysis of HeLa cells using CHD3 antibody [7F6-G2-E5] at a dilution of 1:400.| Application Notes | ICC/IF:1:200~1:500 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | ascites |
