Application
Western blot analysis using anti-CHK2 mAb against cell lysate from various cell types.Application
Confocal immunofluorescence analysis of Hela cells using anti-CHK2 mAb (green), showing nuclear localization. Red: Actin filaments have been labeled with DY-554 phalloidin.Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma (A), liver carcinoma (B), breast carcinoma (C) and kiney carcinoma (D), showing nuclear localization with DAB staining using anti-CHK2 mAb.| Product Name | Chk2 antibody [1C12B8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1C12B8] to Chk2In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarc |
| Immunogen | The immunogen corresponding to a region within amino acids 481 and 531 of human CHK2 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 1C12B8 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHCIHC-PWB |
| ELISA: 1/10000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HCDS1 , CHEK2 , PP1425 , HUCDS1 , CHK2 , CDS1 , LFS2 , RAD53 |
|---|---|
| Target | Chk2 |
| Molecular Weight(MW) | 61 kDa (note) |
| Cellular Localization | Isoform 10 is present throughout the cell. |
Application
Western blot analysis using anti-CHK2 mAb against cell lysate from various cell types.
Application
Confocal immunofluorescence analysis of Hela cells using anti-CHK2 mAb (green), showing nuclear localization. Red: Actin filaments have been labeled with DY-554 phalloidin.
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma (A), liver carcinoma (B), breast carcinoma (C) and kiney carcinoma (D), showing nuclear localization with DAB staining using anti-CHK2 mAb.| Application Notes | ELISA: 1/10000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
