Application
Immunoprecipitation of Chk2 protein from 293T whole cell extracts using 5 μg of Chk2 antibody . Western blot analysis was performed using Chk2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Wild-type (WT) and Chk2 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 antibody [4B8] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
CHEK2 antibody detects CHEK2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CHEK2 antibody diluted at 1:500.| Product Name | Chk2 antibody [4B8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4B8] to Chk2This antibody binds to Chk2. In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. [from RefSeq] |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4B8 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HuCds1, CHK2, PP1425, LFS2, RAD53, CDS1, CHEK2 |
|---|---|
| Target | Chk2 |
Application
Immunoprecipitation of Chk2 protein from 293T whole cell extracts using 5 μg of Chk2 antibody . Western blot analysis was performed using Chk2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Wild-type (WT) and Chk2 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 antibody [4B8] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
CHEK2 antibody detects CHEK2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CHEK2 antibody diluted at 1:500.| Positive Control | 293T , A431 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short term use, store at 4°C. For extended use, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS |
