Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 antibody [8F12] diluted at 1:500.| Product Name | Chk2 antibody [8F12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [8F12] to Chk2CHK2 controls cell cycle checkpoint and may participate in transduction of the DNA damage and replicational stress signals. CHK2 belongs to the Ser/Thr protein kinase family and its kinase actibity is up-regulated by autophosphorylation, It is phosphorylated rapidly in response to DNA damage and to replication block. |
| Immunogen | Recombinant CHK2 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 8F12 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HuCds1, 114480, CHK2, CDS1 CHK2 HuCds1 PP1425 RAD53 bA444G7 checkpoint kinase 2 checkpoint-like protein CHK2 11200, PP1425, CDS1 CHK2 HuCds1 PP1425 RAD53 bA444G7 checkpoint kinase2 checkpointlike protein CHK211200, CDS1 CHK2 HuCds1 PP1425 RAD53 bA444G7 che |
|---|---|
| Target | Chk2 |
| Molecular Weight(MW) | ~61 kDa (note) |
| Tissue Specificity | is specific for human CHK2 protein. |
| Cellular Localization | Nuclear |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 antibody [8F12] diluted at 1:500.| Positive Control | HepG2, MCF-7, or LN-Cap total cell extract |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing no preservatives |
