| Product Name | Cholera Toxin B subunit antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to beta subunit Cholera ToxinCholera toxin (CT) acts by transfer of ADP-ribose from intracellular NAD to the alpha chain of a trimeric G protein that functions to stimulate adenylate cyclase resulting in the increase of host cell cAMP. CT binds via the beta subunits to the GM1 ganglioside. Gangliosides are glycolipids that also contain oligosaccharides with one or more sialic acid residues. |
| Immunogen | Purified Choleragenoid. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC |
| ELISA: Use at a dilution of 1/1000. IHC: Use at an assay dependent dilution. Fluorescence microscopy: Use at an assay dependent dilution. Immunoprecipitates toxin in gel techniques. May be used in place of neat antiserum in almost any appropriate antibody-based technique. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Bacteria |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | Cholera enterotoxin beta chain CTXB TOXB VC1456 |
|---|---|
| Target | Cholera Toxin B subunit |
| Tissue Specificity | recognises the beta subunit of Cholera toxin. |
| Cellular Localization | Secreted |
| Application Notes | ELISA: Use at a dilution of 1/1000. IHC: Use at an assay dependent dilution. Fluorescence microscopy: Use at an assay dependent dilution. Immunoprecipitates toxin in gel techniques. May be used in place of neat antiserum in almost any appropriate antibody-based technique. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 0.01M PBS. pH 7.2 |
