Rabbit polyclonal to Chromogranin AChromogranin A (a protein of 439 amino acid which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.
Immunogen
C-terminal half of Chromogranin A, purified from urine of patients with carcinoid syndrome.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHCIHC-P
IHC-P: 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol with 10 mM citrate buffer, pH 6.0. Optimal dilutions/concentrations should be determined by the end user.
Species Reactivity
Human
Concentration
1mg/ml
Purification
Protein A
Target Information
Gene Synonyms
118910, Chromogranin A, P10645, CGA, 1113, CHGA
Target
Chromogranin A
Cellular Localization
Neuroendocrine and endocrine secretory granules.
Application
Application
IHC Image using - Detection of Chromogranin A by IHC in Human Pancreas
Positive Control
Pancreas
Application Notes
IHC-P: 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol with 10 mM citrate buffer, pH 6.0. Optimal dilutions/concentrations should be determined by the end user.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.