Application
Flow cytometric Analysis of Jurkat cells, using anti-CISD1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CISD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CISD1.Application
Anti-CISD1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CISD1| Product Name | CISD1 antibody [2B3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2B3] to CISD1Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity). May be involved in Fe-S cluster shuttling and/or in redox reactions. |
| Immunogen | Full length human recombinant protein of human CISD1 (NP_060934) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2B3 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| IHC 1:50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC14684, CISD1, MDS029 |
|---|---|
| Target | CISD1 |
| Tissue Specificity | is specific for human CISD1 (NP_060934). |
Application
Flow cytometric Analysis of Jurkat cells, using anti-CISD1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CISD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CISD1.
Application
Anti-CISD1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CISD1| Application Notes | IHC 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
