Application
Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis. A. 50 µg mouse brain lysate/extract 12% SDS-PAGE Citrate synthetase antibody [GT1761] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 12% SDS-PAGE Citrate synthetase antibody [GT1761] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Citrate synthetase antibody detects CS protein at cytosol on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. Citrate synthetase antibody dilution: 1:200.Application
Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis. A. 30 µg zebrafish brain lysate/extract 10% SDS-PAGE Citrate synthetase antibody [GT1761] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Citrate synthetase protein stained by Citrate synthetase antibody [GT1761] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.Application
Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Citrate synthetase antibody [GT1761] dilution: 1:200.Application
Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Citrate synthetase antibody [GT1761] dilution: 1:200.Application
Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Citrate synthetase antibody [GT1761] dilution: 1:200.Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis. A. 30 µg zebrafish heart lysate/extract 10% SDS-PAGE Citrate synthetase antibody [GT1761] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | Citrate synthetase antibody [GT1761] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to Citrate synthetase (citrate synthase)The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Citrate synthetase. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT1761 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRatZebra Fish |
| Predicted Reactivity | Xenopus laevis, Pig, BovineXenopus laevis (91%), Pig (95%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CS antibody, citrate synthase, mitochondrial antibody, citrate synthase antibody |
|---|---|
| Molecular Weight(MW) | 52 kDa (note) (2) |
| Cellular Localization | Mitochondrion matrix |
Application
Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis. A. 50 µg mouse brain lysate/extract 12% SDS-PAGE Citrate synthetase antibody [GT1761] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 12% SDS-PAGE Citrate synthetase antibody [GT1761] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Citrate synthetase antibody detects CS protein at cytosol on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. Citrate synthetase antibody dilution: 1:200.
Application
Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis. A. 30 µg zebrafish brain lysate/extract 10% SDS-PAGE Citrate synthetase antibody [GT1761] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Citrate synthetase protein stained by Citrate synthetase antibody [GT1761] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.
Application
Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Citrate synthetase antibody [GT1761] dilution: 1:200.
Application
Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Citrate synthetase antibody [GT1761] dilution: 1:200.
Application
Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Citrate synthetase antibody [GT1761] dilution: 1:200.
Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis. A. 30 µg zebrafish heart lysate/extract 10% SDS-PAGE Citrate synthetase antibody [GT1761] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , A375 , mouse brain , rat brain , zebrafish brain , zebrafish heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
