Application
Immunofluorescence staining of clathrin in human HeLa cell line using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).Application
Immunofluorescence staining of clathrin in human primary fibroblasts using anti-clathrin (BF-06; green; ). Actin cytoskeleton labeled by phalloidin (red) and cell nuclei stained with DAPI (blue).| Product Name | Clathrin heavy chain antibody [BF-06] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [BF-06] to Clathrin heavy chainClathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.Entrez Gene: 17q11-qter |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | BF-06 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIPWB |
| FACS: Use at 2-5 µg/mL. Positive Control: human blood leukocytes | |
| Species Reactivity | HumanMousePigRatBovine |
| Concentration | 1mg/ml |
| Gene Synonyms | CHC17, CLH-17, CLTCL2, KIAA0034 |
|---|---|
| Target | Clathrin heavy chain |
| Tissue Specificity | The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa protein involved in receptor-mediated endocytosis. |
| Cellular Localization | Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
Application
Immunofluorescence staining of clathrin in human HeLa cell line using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Application
Immunofluorescence staining of clathrin in human primary fibroblasts using anti-clathrin (BF-06; green; ). Actin cytoskeleton labeled by phalloidin (red) and cell nuclei stained with DAPI (blue).| Application Notes | FACS: Use at 2-5 µg/mL. Positive Control: human blood leukocytes |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. Do not freeze. |
| Storage Buffer | Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0 |
