Application
Cell line lysates were separated on SDS-PAGE and probed with 4 μg/mL Monoclonal Anti-Clathrin Heavy Chain Clone: TD.1 . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. SH-SY5Y 4. SK-N-SH 5. COS7 6. PC12 7. Neuro-2a| Product Name | Clathrin Heavy chain antibody [TD.1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [TD.1] to Clathrin heavy chainClathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. |
| Immunogen | Full length native protein (purified) (Cow). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | TD.1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: Use at a concentration of 1 - 4 μg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 192 kDa). Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRatBovine |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | CHC17, CLTCL2, 118955, Q00610, CLTC, 1213, Clathrin Heavy chain, CLH17, CLH-17, KIAA0034, CLH 17 |
|---|---|
| Target | Clathrin Heavy chain |
| Tissue Specificity | Monoclonal Anti-Clathrin Heavy Chain reacts specifically with the clathrin heavy chain (CHC17), and does not cross-react with the muscle isoform CHC22. It is reported to be non-reactive with native clathrin in immunocytochemistry, and immunoprecipitation (but some staining is seen in Rat1 cells, following 4% paraformaldehyde- 0.5% Triton X-100 fixation). |
| Cellular Localization | Cell Membrane and Cytoplasmic |
Application
Cell line lysates were separated on SDS-PAGE and probed with 4 μg/mL Monoclonal Anti-Clathrin Heavy Chain Clone: TD.1 . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. SH-SY5Y 4. SK-N-SH 5. COS7 6. PC12 7. Neuro-2a| Application Notes | WB: Use at a concentration of 1 - 4 μg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 192 kDa). Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
