Application
Immunofluorescent analysis of Clathrin heavy chain in HeLa cells. Clathrin heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Clathrin heavy chain antibody [X22] at a dilution of 1:200 over night at 4 ?C, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.Application
Immunohistochemistry was performed on cancer biopsies of deparaffinized human breast carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with or without Clathrin heavy chain antibody [X22] overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.| Product Name | Clathrin heavy chain antibody [X22] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [X22] to Clathrin heavy chainClathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008] |
| Immunogen | Purified human brain clathrin heavy chain. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | X22 |
| Host Species | Mouse |
| Tested Applications | BlockingELISAFACSICC/IFIHCIHC-PIMIPWB |
| ICC/IF: 1:150 - 1:1000 | |
| Species Reactivity | HumanMouseDogRatHamsterPrimateBovineAvian |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | CLTC , CHC17 , CLTCL2 , clathrin, heavy chain (Hc) , Hc , CHC , CLH-17 |
|---|---|
| Target | Clathrin, Heavy chain |
Application
Immunofluorescent analysis of Clathrin heavy chain in HeLa cells. Clathrin heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Clathrin heavy chain antibody [X22] at a dilution of 1:200 over night at 4 ?C, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.
Application
Immunohistochemistry was performed on cancer biopsies of deparaffinized human breast carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with or without Clathrin heavy chain antibody [X22] overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.| Application Notes | ICC/IF: 1:150 - 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | PBS containing 0.05 % sodium azide |
