Application
Western Blot: CLOCK Antibody - Lanes 3-5 contained alpha CLOCK immunoprecipitated DNA (15, 10 and 5 ul of antisera added respectively to the 900 ul of sonicated chromatin sample in the ChIP assay| Product Name | CLOCK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CLOCKCLOCK, a basic helix-loop-helix (bHLH)/PAS protein, is an important factor in the molecular clock mechanism. Mutations in this protein lead to abnormal circadian behavior and defective transcriptional activity. CLOCK has been shown to interact strongly with other bHLH-PAS proteins such as MOP3, a BMAL1 isoform. |
| Immunogen | Two peptides derived from mouse CLOCK, conjugated to albumin. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CLOCK 12753 601851 KIAA0334 mKIAA0334 5330400M04Rik 9575 O15516 |
|---|---|
| Target | CLOCK |
| Tissue Specificity | This antibody is specific for mCLOCK. |
Application
Western Blot: CLOCK Antibody - Lanes 3-5 contained alpha CLOCK immunoprecipitated DNA (15, 10 and 5 ul of antisera added respectively to the 900 ul of sonicated chromatin sample in the ChIP assay| Application Notes | For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
