Application
Western blot analysis of CLOCK Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). CLOCK (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with CLOCK antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | CLOCK antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CLOCK (Internal)This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 225~255 amino acids from the Center region of human CLOCK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | CLOCK hCLOCK Circadian locomoter output cycles protein kaput KIAA0334 |
|---|---|
| Target | CLOCK |
| Molecular Weight(MW) | 95.304 kDa (note) |
| Cellular Localization | Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent. Phosphorylated form located in the nucleus. |
Application
Western blot analysis of CLOCK Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). CLOCK (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with CLOCK antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
