Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CLPP monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLPP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLPP.Application
Anti-CLPP mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CLPPApplication
HEK293T cells transfected with either RC200301 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CLPP antibody, and then analyzed by flow cytometry.| Product Name | CLPP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1E8] to CLPPClp cleaves peptides in various proteins in a process that requires ATP hydrolysis. Clp may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. |
| Immunogen | Full length human recombinant protein of human CLPP (NP_006003) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1E8 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 : |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | CLPP |
|---|---|
| Tissue Specificity | is specific for human CLPP (NP_006003). |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CLPP monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLPP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLPP.
Application
Anti-CLPP mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CLPP
Application
HEK293T cells transfected with either RC200301 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CLPP antibody, and then analyzed by flow cytometry.| Application Notes | ICC/IF 1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
