Application
c-Met [pYpYpY1230/1234/1235] ELISA Peptide BlockingApplication
Extracts prepared from 293T cells left unstimulated (1) or stimulated with HGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C| Product Name | c-Met (phospho Tyr1230/Tyr1234/Tyr1235) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Met ( c - Met ) ( phospho Y1230 + Y1234 + Y1235 )Binding of scatter factor (SF)/hepatocyte growth factor (HGF) to the c Met receptor tyrosine kinase (RTK) triggers receptor dimerization and phosphorylation on multiple residues within the juxtamembrane, catalytic core and cytoplasmic tail domains, thereby regulating receptor internalization, catalytic activity and multisubstrate docking. c Met contains three tyrosines (Tyr-xx- x-Tyr-Tyr motif) within the activation loop of the catalytic domain. This is also seen with the insulin receptor, insulin-like growth factor receptor (IGF1) receptor and nerve growth factor (NGF) receptors/Trks, for which phosphorylation of all three tyrosines is required for full activation. With c Met (and the related family member, RON) phosphorylation of tyrosines 1234 and 1235 has been shown to be important in receptor activation. Activation of the c Met receptor results in binding and/or phosphorylation of many intracellular signaling proteins including multiple adaptor proteins (e.g., Grb2, Shc, Cbl, Crk, cortactin, paxillin, and GAB1), and a variety of other signal transducers (e.g., PI 3-kinase, FAK, Src, Erk1&2, JNK, PLC-?, and STAT3). |
| Immunogen | Synthetic phosphopeptide derived from the region of human c-Met that contains tyrosines 1230, 1234 and 1235. |
| Modification | p-Tyr1230/Tyr1234/Tyr1235 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB: Use at a dilution of 1:1000. Detects a band of approximately 169 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | c Met cMet AUTS9 HGFR RCCP2 c-Met c-Met (phospho Y1230/Y123) c-Met (phospho Tyr1230/Tyr123) c-Met (pY1230/pY123) c-Met (pTyr1230/pTyr123) c-Met phospho Y1230/Y123 c-Met phospho Tyr1230/Tyr123 Phospho c-Met (Y1230/Y123) Phospho c-Met (Tyr1230/T |
|---|---|
| Target | c-Met |
| Tissue Specificity | The phosphospecific antibody that has been generated does not distinguish between the dually (pYpY 1234/1235) and triply (pYpYpY1230/1234/1235) phosphorylated forms of c-Met, both of which are likely to represent activated forms of this receptor. |
| Cellular Localization | Type I membrane protein |
Application
c-Met [pYpYpY1230/1234/1235] ELISA Peptide Blocking
Application
Extracts prepared from 293T cells left unstimulated (1) or stimulated with HGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C| Positive Control | 293T human kidney cells transiently transfected with human Met cDNA, stimulated with HGF.Mouse myeloma (SP-1) cells stimulated with HGF. A431 cells stimulated with EGF |
|---|---|
| Application Notes | WB: Use at a dilution of 1:1000. Detects a band of approximately 169 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS, 1mg/ml BSA |
