Application
Extracts of H69 cells unstimulated (lane 1) or treated with 40 ng/mL HGF for 7.5 minutes (lanes 2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer.| Product Name | c-Met (phospho Tyr1003) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Met ( c - Met ) ( phospho Y1003 )Binding of scatter factor (SF)/hepatocyte growth factor (HGF) to the c Met receptor tyrosine kinase (RTK) triggers receptor dimerization and phosphorylation on multiple residues within the juxtamembrane, catalytic core and cytoplasmic tail domains, thereby regulating receptor internalization, catalytic activity and multisubstrate docking. c Met contains three tyrosines (Tyr-xx- x-Tyr-Tyr motif) within the activation loop of the catalytic domain. This is also seen with the insulin receptor, insulin-like growth factor receptor (IGF1R) and nerve growth factor (NGF) receptors/Trks, for which phosphorylation of all three tyrosines is required for full activation. With c Met (and the related family member, RON) phosphorylation of Tyr1234 and Tyr1235 have been shown to be important in receptor activation. Activation of the c Met receptor results in binding and/or phosphorylation of many intracellular signaling proteins including multiple adaptor proteins (e.g., Grb2, Shc, Cbl, Crk, cortactin, paxillin, and GAB1), and a variety of other signal transducers (e.g., PI 3-kinase, FAK, Src, ERK1&2, JNK1&2, PLC gamma, and STAT3). Tyr1003 is a negative regulatory phosphorylation site of c Met. |
| Immunogen | Synthetic phosphopeptide derived from the region of c-Met that contains tyrosine 1003. |
| Modification | p-Tyr1003 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1/1000. Detects a band of approximately 145 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | c Met cMet c-Met (phospho Y1003) c-Met (phospho Tyr1003) c-Met (pY1003) c-Met (pTyr1003) c-Met phospho Y1003 c-Met phospho Tyr1003 Phospho c-Met (Y1003) Phospho c-Met (Tyr1003) Phospho c-Met (pY1003) Phospho c-Met (pTyr1003) |
|---|---|
| Target | c-Met |
| Cellular Localization | Type I membrane protein |
Application
Extracts of H69 cells unstimulated (lane 1) or treated with 40 ng/mL HGF for 7.5 minutes (lanes 2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer.| Positive Control | 293T cells transiently transfected with human c-Met and stimulated with HGF |
|---|---|
| Application Notes | WB: 1/1000. Detects a band of approximately 145 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 1mg/ml BSA. pH 7.4 |
