| Product Name | c-Met (phospho Tyr1365) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Met ( c - Met ) ( phospho Y1365 )Binding of scatter factor (SF)/hepatocyte growth factor (HGF) to the c Met receptor tyrosine kinase (RTK) triggers receptor dimerization and phosphorylation on multiple residues within the juxtamembrane, catalytic core and cytoplasmic tail domains, thereby regulating receptor internalization, catalytic activity and multisubstrate docking. c Met contains three tyrosines (Tyrx- x-x-Tyr-Tyr motif) within the activation loop of the catalytic domain. This is also seen with the insulin receptor, insulin-like growth factor receptor (IGF1R) and nerve growth factor (NGF) receptors/Trks, for which phosphorylation of all three tyrosines is required for full activation. With c Met (and the related family member, RON) phosphorylation of Tyr1234 and Tyr1235 have been shown to be important in receptor activation. Activation of the c Met receptor results in binding and/or phosphorylation of many intracellular signaling proteins including multiple adaptor proteins (e.g., Grb2, Shc, Cbl, Crk, cortactin, paxillin, and GAB1), and a variety of other signal transducers (e.g., PI 3-kinase, FAK, Src, ERK1&2, JNK1&2, PLC gamma, and STAT3). The phosphorylation of Tyr1365 has been shown to inhibit cell morphogenesis. |
| Immunogen | Synthetic phosphopeptide (Human) derived from the region of c Met that contains tyrosine 1365. |
| Modification | p-Tyr1365 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1 - 1.0 μg/ml. Detects a band of approximately 169 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | c Met cMet AUTS9 HGFR RCCP2 c-Met c-Met (phospho Y1365) c-Met (phospho Tyr1365) c-Met (pY1365) c-Met (pTyr1365) c-Met phospho Y1365 c-Met phospho Tyr1365 Phospho c-Met (Y1365) Phospho c-Met (Tyr1365) Phospho c-Met (pY1365) Phospho c-Met (pTy |
|---|---|
| Target | c-Met |
| Cellular Localization | Type I membrane protein |
| Positive Control | 293T cells transiently transfected with human c-Met and stimulated with HGF |
|---|---|
| Application Notes | WB: Use at a concentration of 0.1 - 1.0 μg/ml. Detects a band of approximately 169 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 1mg/ml BSA. pH 7.4 |
