Application
Cofilin 1 antibody [GT567] detects CFL1 protein by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract B. 30 µg JC whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract 12% SDS-PAGE Cofilin 1 antibody [GT567] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Cofilin 1 antibody [GT567] detects CFL1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 12% SDS-PAGE Cofilin 1 antibody [GT567] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Cofilin 1 antibody [GT567] detects Cofilin 1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: Cofilin 1 protein stained by Cofilin 1 antibody [GT567] diluted at 1:300. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Cofilin 1 antibody [GT567] detects CFL1 protein at cytosol on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. Cofilin 1 antibody [GT567] dilution: 1:200.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | Cofilin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to Cofilin 1 (cofilin 1 (non-muscle))Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cofilin 1. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT567 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Sheep, Rhesus Monkey, BovineRat (99%), Pig (99%), Sheep (99%), Rhesus Monkey (99%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Molecular Weight(MW) | 19 kDa (note) |
|---|---|
| Cellular Localization | Nucleus matrix , Cytoplasm , cytoskeleton |
Application
Cofilin 1 antibody [GT567] detects CFL1 protein by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract B. 30 µg JC whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract 12% SDS-PAGE Cofilin 1 antibody [GT567] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Cofilin 1 antibody [GT567] detects CFL1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 12% SDS-PAGE Cofilin 1 antibody [GT567] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Cofilin 1 antibody [GT567] detects Cofilin 1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: Cofilin 1 protein stained by Cofilin 1 antibody [GT567] diluted at 1:300. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Cofilin 1 antibody [GT567] detects CFL1 protein at cytosol on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. Cofilin 1 antibody [GT567] dilution: 1:200.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , A375 , NIH-3T3 , JC , BCL-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
