Application
COL1A2 antibody [C2C3], C-term detects COL1A2 protein at cytoplasm by immunofluorescent analysis. Sample: NIH-3T3 cells were fixed in ice-cold MeOH for 5 min. Green: COL1A2 protein stained by COL1A2 antibody [C2C3], C-term diluted at 1:100. Blue: Hoechst 33342 staining.| Product Name | COL1A2 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to COL1A2 (collagen, type I, alpha 2)This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human COL1A2. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500~1:2000 ICC/IF:1:50~1:100 |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Rabbit, Chicken, Rhesus Monkey, BovineRat (88%), Dog (95%), Pig (95%), Rabbit (90%), Chicken (82%), Rhesus Monkey (100%), Bovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | OI4 antibody COL1A2 antibody collagen I alpha-2 polypeptide antibody collagen of skin tendon and bone alpha-2 chain antibody type I procollagen antibody collagen alpha-2(I) chain antibody alpha 2(I)-collagen antibody alpha-2 type I collagen antib |
|---|---|
| Molecular Weight(MW) | 129 kDa (note) |
| Cellular Localization | Secreted , extracellular space , extracellular matrix |
Application
COL1A2 antibody [C2C3], C-term detects COL1A2 protein at cytoplasm by immunofluorescent analysis. Sample: NIH-3T3 cells were fixed in ice-cold MeOH for 5 min. Green: COL1A2 protein stained by COL1A2 antibody [C2C3], C-term diluted at 1:100. Blue: Hoechst 33342 staining.| Positive Control | GFP-human COL1A2-transfected 293T cells (partial) |
|---|---|
| Application Notes | WB:1:500~1:2000 ICC/IF:1:50~1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
