| Product Name | Collagen II antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Collagen IICollagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein. Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. |
| Immunogen | Collagen Type II extracted and purified from rat fetal cartilage. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-P |
| ELISA: Use diluted at > 1:100 – 1:200 (OD > 500). IF: 1/80. IHC-P : Use diluted at > 1:250 on fixed paraffin-embedded rat cartilage tissues. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | Col2a1 Collagen II 25412 CG2A1A COLLII |
|---|---|
| Target | Collagen II |
| Tissue Specificity | This antibody is specific to Rat Collagen Type II (100%) by RIA, and shows neglibible reactivity with Rat Collagen Type I ( |
| Cellular Localization | Secreted |
| Positive Control | Frozen rat tissue sections for indirect IFA |
|---|---|
| Application Notes | ELISA: Use diluted at > 1:100 – 1:200 (OD > 500). IF: 1/80. IHC-P : Use diluted at > 1:250 on fixed paraffin-embedded rat cartilage tissues. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
