| Product Name | Collagen II antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Collagen IICollagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. |
| Immunogen | Collagen Type II extracted and purified from bovine cartilage. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFRIA |
| ELISA: Use at an assay dependent dilution. IF: 1/40. RIA: 1/100. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Bovine |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | Collagen II 282189 COL2A1 |
|---|---|
| Target | Collagen II |
| Tissue Specificity | This antibody is specific to Bovine Collagen Type II (100%) when tested by RIA, and shows typically less than 3% reactivity against other Bovine Collagen Types (IX and XI). |
| Cellular Localization | Secreted |
| Positive Control | Frozen bovine articular cartilage for indirect IFA |
|---|---|
| Application Notes | ELISA: Use at an assay dependent dilution. IF: 1/40. RIA: 1/100. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
