WB
Western blot analysis of lysates from 1) k562 , 2) COLO205,3) MCF-7 cells, (Green) primary antibody was diluted at 1:1000, 4℃ over night, secondary antibody was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody antibody was diluted at 1:5000 as loading control, 4℃ over night,secondary antibody was diluted at 1:10000, 37° 1hour.IHC
Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,COL2A1 Polyclonal Antibody was diluted at 1:200 (4℃ overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98℃,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.IHC
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,COL2A1 Polyclonal Antibody was diluted at 1:200 (4℃ overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98℃,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.IHC
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,COL2A1 Polyclonal Antibody was diluted at 1:200 (4℃ overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98℃,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.IF-P
Immunofluorescence analysis of rat-spleen tissue. 1,COL2A1 Polyclonal Antibody (red) was diluted at 1:200 (4℃ overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+BICC/IF
Immunofluorescence analysis of COS7 cells, using Collagen II Antibody. The picture on the right is blocked with the synthesized peptide.| Product Name | COL2A1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Collagen II. AA range:101-150 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COL2A1 |
|---|---|
| Gene Synonyms | AOM ACG2 ANFH LCPD SEDC STL1 VPED ANFH1 EDMMD OSCDP PLSDT SEDSTN SMDALG COL11A3 SEMDSTWK |
| Gene Full Name | collagen type II alpha 1 chain |
| Gene Summary | This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | COL2A1 Collagen alpha-1 II chain Alpha-1 type II collagen |
| Molecular Weight(MW) | 140 kDa |
| Cellular Localization | Secreted , extracellular space , extracellular matrix . |
WB
Western blot analysis of lysates from 1) k562 , 2) COLO205,3) MCF-7 cells, (Green) primary antibody was diluted at 1:1000, 4℃ over night, secondary antibody was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody antibody was diluted at 1:5000 as loading control, 4℃ over night,secondary antibody was diluted at 1:10000, 37° 1hour.
IHC
Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,COL2A1 Polyclonal Antibody was diluted at 1:200 (4℃ overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98℃,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
IHC
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,COL2A1 Polyclonal Antibody was diluted at 1:200 (4℃ overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98℃,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
IHC
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,COL2A1 Polyclonal Antibody was diluted at 1:200 (4℃ overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98℃,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
IF-P
Immunofluorescence analysis of rat-spleen tissue. 1,COL2A1 Polyclonal Antibody (red) was diluted at 1:200 (4℃ overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
ICC/IF
Immunofluorescence analysis of COS7 cells, using Collagen II Antibody. The picture on the right is blocked with the synthesized peptide.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
