WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Collagen II antibody. The HRP conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C-28/I2, Lane 2: SW1353, Lane 3: A431.IHC
Human cartilage was stained with anti-Collagen II rabbit antibody.| Product Name | Collagen II Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:5000 IHC:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | COL2A1 |
|---|---|
| Gene Synonyms | AOM ACG2 ANFH LCPD SEDC STL1 VPED ANFH1 EDMMD OSCDP PLSDT SEDSTN SMDALG COL11A3 SEMDSTWK |
| Gene Full Name | collagen type II alpha 1 chain |
| Gene Summary | This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 142kDa(Observed band size: 200kDa) |
| Cellular Localization | Extracellular matrix,Secreted. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Collagen II antibody. The HRP conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C-28/I2, Lane 2: SW1353, Lane 3: A431.
IHC
Human cartilage was stained with anti-Collagen II rabbit antibody.| Application Notes | WB:1:500-1:5000 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
