Application
Staining of frozen rat skin sections with 1:8,000 Monoclonal Anti Collagen, Type III Clone: FH-7A (Cat. No. ) using biotin/ExtrAvidin®-Peroxidase.Application
Staining of formalin-fixed, paraffin-embedded rat skin with 1:4,000 Monoclonal Anti Collagen, Type III Clone: FH-7A (Cat. No. ) using biotin/ExtrAvidin®-Peroxidase.| Product Name | Collagen III antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [FH - 7A] to Collagen IIICollagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in the gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons. |
| Immunogen | full length native protein human collagen type III |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | FH-7A |
| Host Species | Mouse |
| Tested Applications | DotELISAIHC-FIHC-PWB |
| For IHC-Fr: Use at a dilution of 1:4,000. WB: 1/100 - 1/1000. The antibody recognizes a ~ 70 kDa band in reduced denatured preps and a ~ 300 kDa band in non-reduced, non-denatured preps. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Collagen III EDS4A P02461 1281 120180 COL3A1 |
|---|---|
| Target | Collagen III |
| Tissue Specificity | The antibody specifically recognizes native and denatured collagen type III. It does not recognise collagen types I, II, IV, V, VI and X. |
| Cellular Localization | Extracellular matrix |
Application
Staining of frozen rat skin sections with 1:8,000 Monoclonal Anti Collagen, Type III Clone: FH-7A (Cat. No. ) using biotin/ExtrAvidin®-Peroxidase.
Application
Staining of formalin-fixed, paraffin-embedded rat skin with 1:4,000 Monoclonal Anti Collagen, Type III Clone: FH-7A (Cat. No. ) using biotin/ExtrAvidin®-Peroxidase.| Positive Control | IHC: Human skin sections WB: recombinant collagen III |
|---|---|
| Application Notes | For IHC-Fr: Use at a dilution of 1:4,000. WB: 1/100 - 1/1000. The antibody recognizes a ~ 70 kDa band in reduced denatured preps and a ~ 300 kDa band in non-reduced, non-denatured preps. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. For prolonged storage, freeze in working aliquots. Avoid repeated freezing and thawing. |
| Storage Buffer | Contains 15 mM sodium azide |
